Ccar1 Knockout cell line (E0771)

Loci	STR Info (Sample Cell) Sample Cell Line:E0771				STR Info (Cell bank) Cell Line:EO771
Loci	Allele1	Allele2	Allele3	Allele4	Allele1	Allele2	Allele3	Allele4
18-3	16.0	17.0	18.0		16.0	17.0	18.0
4-2	21.3	22.3	24.3		21.3	22.3	24.3
6-7	15.0	16.0			15.0	16.0
19-2	13.0				13.0
1-2	19.0	20.0	22.0		19.0	20.0	22.0
7-1	25.2	26.2			25.2	26.0	26.2
8-1	16.0				16.0
1-1	17.0	18.0	19.0		17.0	18.0	19.0
3-2	14.0	15.0			14.0	15.0
2-1	16.0	18.0			16.0	18.0
15-3	22.3	23.3	25.3		22.3	23.3
6-4	18.0	19.0	20.0		18.0	19.0	20.0	21.0
13-1	17.1	18.1			17.0	18.0
11-2	15.3	16.3			15.3	16.3
17-2	14.0	15.0	16.0		15.0	16.0	17.0
12-1	17.0	18.0			17.0	18.0
5-5	17.0	21.0			17.0	21.0	22.0
X-1	26.0	29.0			27.0	29.0
TH01
D5S818

* STR authentication data of this cell line matches with that of cell lines sourced from ATCC, DSMZ, JCRB, and RIKEN databases.
Conclusion: The STR identification of this cell is correct.

Instruction

Use exclusive Red Cotton system to design a sgRNA fragment knockout strategy for the Ccar1 gene, clone the two sgRNAs into CRISPR-U™ vector, transfect the vector into the E0771 cell line by electroporation/lentivirus method, use the limited dilution method for single-cell cloning and use the EZ-editor™ Monoclone Genotype Validation Kit (Cat# YK-MV-1000) for nucleic acid lysis and PCR amplification on the single-cell clones, followed by Sanger sequencing to verify the genotype. Upon passing the verification, Ccar1 gene knockout Ccar1 Knockout cell line（E0771） will be expanded and cryopreserved.

Knockout Cell Line Work Flow and Validatio

Ccar1 Knockout cell line (E0771)

Catalog#

YKO-M235

Size

1*10^6

Storage conditions

Shipped on dry ice; Store in liquid nitrogen

Order

Related Popular Products

Wild-type Cell Lines

Murine Medullary Breast Cancer Cell Line(E0771)
inquiry More details

Catalog#: YC-C034

Size: 1*10^6

Instruction:

Gene Knockout kit

EZ-editor™Gene Knockout kit
inquiry More details

Catalog#: MY-001

Size: -

Instruction:

EZ-cryo™ cell freezing medium

EZ-cryo™ Cell Freezing Medium
inquiry More details

Catalog#: YK-CR-50;YK-CR-100

Size: 50ml;100ml

Instruction:

KO Cell Lines

CCAR1 Knockout cell line (Hep G2)
inquiry More details

Organism: Human

Gene Name: CCAR1

Gene ID: 55749

Size: 1*10^6

Catalog#: YKO-HT2748

YKO-HT2748(Hep G2)
Ccar1 Knockout cell line (4T1)
inquiry More details

Organism: Mouse

Gene Name: Ccar1

Gene ID: 67500

Size: 1*10^6

Catalog#: YKO-M184

YKO-M184(4T1)
CCAR1 Knockout cell line (HeLa)
inquiry More details

Organism: Human

Gene Name: CCAR1

Gene ID: 55749

Size: 1*10^6

Catalog#: YKO-HS09184

YKO-HS09184(HeLa)

Related Popular Products

Wild-type Cell Lines

Murine Medullary Breast Cancer Cell Line(E0771)

Price (USD): -

Catalog#: YC-C034

Size: 1*10^6

Instruction:

inquiry More details

Gene Knockout kit

EZ-editor™Gene Knockout kit

Price (USD): 998

Catalog#: MY-001

Size: -

Instruction:

inquiry More details

EZ-cryo™ cell freezing medium

EZ-cryo™ Cell Freezing Medium

Price (USD): -;-

Catalog#: YK-CR-50;YK-CR-100

Size: 50ml;100ml

Instruction:

inquiry More details

KO Cell Lines

CCAR1 Knockout cell line (Hep G2)

Organism: Human

Gene Name: CCAR1

Gene ID: 55749

Size: 1*10^6

Catalog#: YKO-HT2748

inquiry More details
Ccar1 Knockout cell line (4T1)

Organism: Mouse

Gene Name: Ccar1

Gene ID: 67500

Size: 1*10^6

Catalog#: YKO-M184

inquiry More details
CCAR1 Knockout cell line (HeLa)

Organism: Human

Gene Name: CCAR1

Gene ID: 55749

Size: 1*10^6

Catalog#: YKO-HS09184

inquiry More details

Ccar1 Knockout cell line (E0771)

Subscribe Us